The Horiba MacroRAM is a compact Raman benchtop system with a probe attachment and manual focusing stage. We acquired the data using the Horiba LabSpec 6 software.
| - | Acetonitrile | Chlamydomonas cells |
|---|---|---|
| Wavelength | 785 nm | 785 nm |
| Power | 60 mW | 50 mW |
| Exposure | 10 s | 10 s |
| Num averages | 5 | 5 |
The Renishaw inVia Qontor is a confocal Raman system built on a Leica microscope and equipped with an automated mapping stage. We recorded spectra of acetonitrile and Chlamydomonas using a 5x and a 50x LWD objective, respectively. We prepared slides of Chlamydomonas and focused using the built-in camera. We acquired the data using the Renishaw WiRE software.
| - | Acetonitrile | Chlamydomonas cells |
|---|---|---|
| Wavelength | 785 nm | 785 nm |
| Power | Not measured | 16.3 mW |
| Exposure | 10 s | 5–10 s |
| Num averages | 1 | 5–20 |
The Wasatch WP 785X is a compact system with a probe attachment, integrated illumination source, and no stage. We built a holder and stage for this system. We acquired the data using the Wasatch Photonics ENLIGHTEN software (version 4.1.6).
| - | Acetonitrile | Chlamydomonas cells |
|---|---|---|
| Wavelength | 785 nm | 785 nm |
| Power | 100 mW | 50 mW |
| Exposure | 1 s | 3 s |
| Num averages | 2 | 2 |
The Wasatch WP 532X is a compact system with an external laser (Cobolt), a probe attachment, and no stage. We built a holder and stage for this system. We acquired the data using the Wasatch Photonics ENLIGHTEN software (version 4.1.6).
| - | Acetonitrile | Chlamydomonas cells |
|---|---|---|
| Wavelength | 532 nm | 532 nm |
| Power | 5 mW | 5 mW |
| Exposure | 1 s | 1 s |
| Num averages | 1 | 2 |
The OpenRAMAN starter edition is a low-cost, open-source Raman spectroscopy system developed by Luc Boussemaere. We have previously used and described this system in our research (Avasthi et al., 2024; Braverman et al., 2025), but we’ve modified it to achieve higher throughput by adding a motorized stage. We acquired the data using the Spectrum Analyzer Suite (version 1.0.1) and µManager (version 2.0.3).
| - | Acetonitrile | Chlamydomonas cells |
|---|---|---|
| Wavelength | 532 nm | 532 nm |
| Power | 4.5 mW | 4.5 mW |
| Exposure | 10 s | 5 s |
| Num averages | 5 | 2 |